[摘要]目的：研究外源性透明质酸对创面愈合组织中生化组分的影响，以期探索外源性HA在创伤愈合中的作用及其机制。方法：在小型猪背部的去中厚皮创面上应用不同浓度和剂型的外源性HA，并于术后第3、7、14、21、28天切取创面组织及正常皮肤，测定其糖胺多糖、HA及Ⅲ型胶原的含量变化。结果：愈合过程中，实验组创面愈合组织中的GAG、HA含量逐渐升高，1周时达峰值并能维持3周；创面对照组术后3天也见增高，但随后即迅速下降。实验组的Ⅲ型胶原含量也始终明显高于对照组，其中又以1.0%、2.0%HA组最高。结论：外源性HA能显著增高愈合组织中HA、Ⅲ型胶原含量并能维持较长时间，从而有利于创伤愈合及减轻瘢痕形成。
[关键词]透明质酸;创面愈合;糖胺多糖
[中图分类号]R641 [文献标识码]A [文章编号]1008-6455(2003)06-0573-03
The effect of hyaluronan on matrix changement in healing wounds
HE Zhen-ping1, CHENG Ning-xin1, WANG Yuan-lu1, LIU Xiao-ting1 SUN Guang-ci2 and Guan Zheng-yu2
(1.Department of Plastic Surgery the First People's Hospital of Guangzhou ,guangdong 510180 ,China;2. The Union Medical University of China )
Abstract: Objective This study is to elucidate the change of ECM in skin wound after using exogenous HA, and its underlying mechanisms in scarless-healing. Methods After having made excisional skin-wound on backs of iglets, we used exogenous HA gelon wounds. Then we tested the contents of GAG ,HA and collagen Ⅲ in wounds andnormal skin. Results GAG and HA contents in experimental wounds were gradually increased to its peak in a week and kept it for 3weeks, the controls increased on the 3rd day and then decreased rapidly. So as the change of collagen Ⅲ in this study. Conclusion Using exogenous HA on skin wounds of piglets is able to raise HA and collagen Ⅲ contents in healing-wounds and keeps it for more than 3 weeks. So exogenous HA can accelerate wound healing and alleviate scar formation.
Key words: hyaluronic acid；wound healing； GAG
减小字体
增大字体